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2025
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2025
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2025
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2025
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2025
ABSTRACTMitochondria play a fundamental role in energy metabolism, particularly in high‐energy‐demand tissues such as skeletal muscle. Understanding the proteomic composition of mitochondria in these cells is crucial for elucidating the mechanisms underlying muscle physiology and pathology. However, effective isolation of mitochondria from primary human skeletal muscle cells has been challenging due to the complex cellular architecture and the propensity for contamination with other organelles. Here, we compared four different methods to isolate mitochondria from primary human skeletal myoblasts regarding total protein yield, mitochondrial enrichment capacity and purity of the isolated fraction. We presented a modified method that combines differential centrifugation with a hypotonic swelling step and a subsequent purification process to minimise cellular contamination. We validated our method by demonstrating its ability to obtain highly pure mitochondrial fractions, as confirmed by Western Blot with mitochondrial, cytosolic and nuclear markers. We demonstrated that proteomic analysis can be performed with isolated mitochondria. Our approach provides a valuable tool for investigating mitochondrial dynamics, biogenesis and function in the context of skeletal muscle biology in health and disease. This methodological advancement opens new avenues for mitochondrial research and its implications in myopathies, sarcopenia, cachexia and metabolic disorders.- Book : 29(2)
- Pub. Date : 2025
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2025
Study Design Systematic review. Objective In patients with extradural metastatic spine disease, we sought to systematically review the outcomes and complications of patients with intermediate Spinal Instability Neoplastic Score (SINS) lesions undergoing radiation therapy, percutaneous interventions, minimally invasive surgeries, or open spinal surgeries. Methods Following PRISMA guidelines for systematic reviews, MEDLINE, EMBASE, Web of Science, the Cochrane Database of Systematic Reviews and the Cochrane Center Register of Controlled Trials were queried for studies that reported on SINS intermediate patients who underwent: 1) radiotherapy, 2) percutaneous intervention, 3) minimally invasive, or 4) open surgery. Dates of publication were between 2013-22. Patients with low- or high-grade SINS were excluded. Outcome measures were pain score, functional status, neurological outcome, ambulation, survival, and perioperative complications. Results Thirty-nine studies (n = 4554) were included that analyzed outcomes in the SINS intermediate cohort. Radiotherapy appeared to provide temporary improvement in pain score; however, recurrent pain led to surgery in 15%–20% of patients. Percutaneous vertebral augmentation provided improvement in pain. Minimally invasive surgery and open surgery offered improvement in pain, quality of life, neurological, and ambulatory outcomes. Open surgery may be associated with more complications. There was limited evidence for radiofrequency ablation. Conclusion In the SINS intermediate group, radiotherapy was associated with temporary improvement of pain but may require subsequent surgery. Both minimally invasive surgery and open spinal surgery achieved improvements in pain, quality of life, and neurological outcomes for patients with spine metastases. Open surgery may be associated with more complications. - Book : 15(1_suppl)
- Pub. Date : 2025
- Page : pp.132S-142S
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2025
The observation-based analysis of drought development in the Uttar Pradesh region in India showed that, despite the area being relatively large, agricultural drought exhibits high spatial variability. However, the lack of net radiation data hinders the capacity to provide reliable estimates of evapotranspiration (ET), affecting the assessment of drought occurrence since its propagation across the hydrological system becomes very sensitive to the estimation of ET. The most prominent precipitation deficits occur during the monsoon season (June to October), showing that changes in the large-scale circulation are responsible for the impact of severe drought. El Niño-Southern Oscillation (ENSO) modulates the variability of drought with a warm phase favoring drought development with the strongest influence between August and October. The climate change projections under RCP4.5 and RCP8.5 scenarios suggest the intensification of drought events in the Uttar Pradesh region in the mid-century, with the Chambal River of the Ganges River basin being the most affected area in terms of precipitation and temperature. The projected scenarios correspond to an increase of 1.7°C for mean temperature, and 3.5°C for minimum and maximum temperature in the 2050 horizon, and a decrease of 400 to 800 mm for annual precipitation was projected under both RCPs.- Book : ()
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2025
Acetic acid bacteria (AAB) are versatile organisms that catalyze the conversion of a wide range of carbon sources into biomolecules of great industrial interest. In this study, we exploited the ability of Gluconobacter spp. to synthesize levans. Among the isolated strains, Gluconobacter cerinus UELBM11 produced approximately 14.0 g L-1 of levan under non-optimized conditions. Gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) analyses confirmed that levan obtained from G. cerinus UELBM11 consisted of a β-(2→6)-D-fructose backbone with some β-(2→1) ramifications. The average molecular weight (Mw) of the purified levan was 8.78 × 105 Da. Thermogravimetric/differential thermogravimetric (TGA/DTG) analysis indicated high thermal stability, with the maximum decomposition rate observed at 227.44 °C. Scanning electron microscopy (SEM) revealed a microporous morphology, and the antioxidant activity assays demonstrated that levan had a high scavenging capacity of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radicals. Therefore, it has been demonstrated the levan produced by G. cerinus UELBM11 is a promising natural antioxidant and, owing to its microporosity and excellent thermal properties and stability, is a potential candidate as an additive in cosmetics, pharmaceuticals, and food products.- Book : ()
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2025
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Abstract
We explore the gravitational properties of a nonlinear electromagnetic extension of an AdS Reissner-Nordström black hole. Our study begins with an analysis of the metric function and horizon structure, followed by calculations of the Ricci and Kretschmann scalars and an evaluation of the non-vanishing Christoffel symbols. These calculations allow us to examine geodesics and their influence on the photon sphere and shadow formation. In the thermodynamic framework, we evaluate essential quantities, including the Hawking temperature, entropy, heat capacity, Gibbs free energy, and Hawking radiation emission. We further investigate black hole evaporation by estimating the evaporation timescale as the black hole approaches its final state. Additionally, quasinormal modes for scalar and vector perturbations are computed using the WKB approximation to characterize oscillatory behavior of the system. Finally, a time-domain analysis is provided in order to examine the evolution of these perturbations.- Book : 2025(01)
- Pub. Date : 2025
- Page : pp.072-072
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