• Book : 36(1)
• Pub. Date : 2025
• Page : pp.16
• Keyword :

• Book : 18(1)
• Pub. Date : 2025
• Page : pp.101272
• Keyword :

• Book : 18(1)
• Pub. Date : 2025
• Page : pp.101275
• Keyword :

• Book : 18(1)
• Pub. Date : 2025
• Page : pp.101241
• Keyword :

• Book : 18(1)
• Pub. Date : 2025
• Page : pp.101266
• Keyword :

Objective

Antinuclear antibodies (ANA) staining patterns can provide useful information in systemic lupus erythematosus (SLE). In our study, we examined the frequency of ANA staining patterns in disease-related features in childhood-onset SLE patients.

Methods

ANA and its staining patterns were assessed in childhood-onset SLE patients.

Results

Two hundred twenty-three patients were included (F/M = 3/1). Their median age at diagnosis was 14.3 (11.9-16.1) years. The anti-cell (AC)-4/5 (fine or large speckled) pattern was the most common nuclear ANA pattern (75.8%), while the AC-19 (dense fine speckled) pattern was the most frequently detected cytoplasmic ANA pattern (13.1%). The AC-4/5 (fine or large speckled) patterns were notably seen in fever, acute and chronic cutaneous lupus, arthritis, serositis, hematologic involvement, renal involvement, neuropsychiatric involvement, gastrointestinal involvement, and cardiopulmonary involvement (all p < .001). Conversely, the AC-1 (homogeneous) pattern was significantly detected in oral/nasal ulcers and non-scarring alopecia (both p < .001). Regarding the laboratory features, the AC-4/5 (fine or large speckled) patterns exhibited a predominant seen in autoimmune hemolytic anemia, leukopenia, thrombocytopenia, elevated ESR and CRP, hypocomplementemia, direct Coombs, anti-Smith (Sm), anti-SSA and SS-B, anti-ribonucleoprotein (RNP), anti-histone, anti-ribosomal P, lupus anticoagulant, anti-cardiolipin immunoglobulin (Ig)M/IgG, and anti-β2-glycoprotein IgM/IgG positivities (all p < .001). In contrast, the AC-1 (homogeneous) pattern was detected in anti-double-stranded (ds) DNA and anti-histone positivity (both p < .001).

Conclusion

Our study showed that AC-4/5 and AC-1 patterns of ANA are frequently detected in many clinical and serological features of childhood-onset SLE patients. However, further studies are needed in larger populations to verify these results.

• Book : 34(1)
• Pub. Date : 2025
• Page : pp.71-78
• Keyword :

• Book : 132()
• Pub. Date : 2025
• Page : pp.110983
• Keyword :

• Book : 114(1)
• Pub. Date : 2025
• Page : pp.434-448
• Keyword :

Summary

Adaptive radiation in Ophrys orchids leads to complex floral phenotypes that vary in scent, color and shape.

Using a novel pipeline to quantify these phenotypes, we investigated trait divergence at early stages of speciation in six populations of Ophrys aveyronensis experiencing recent allopatry. By integrating different genetic/genomic techniques, we investigated: variation and integration of floral components (scent, color and shape); phenotypes and genomic regions under divergent selection; and the genomic bases of trait variation.

We identified a large genomic island of divergence, likely associated with phenotypic variation in particular in floral odor. We detected potential divergent selection on macular color, while stabilizing selection was suspected on floral morphology and for several volatile olfactive compounds. We also identified candidate genes involved in anthocyanin and in steroid biosynthesis pathways associated with standing genetic variation in color and odor.

This study sheds light on early differentiation in Ophrys, revealing patterns that often become invisible over time, that is the geographic mosaic of traits under selection and the early appearance of strong genomic divergence. It also supports a crucial genomic region for future investigation and highlights the value of a multifaceted approach in unraveling speciation within taxa with large genomes.

• Book : 245(2)
• Pub. Date : 2025
• Page : pp.849-868
• Keyword :

• Book : 11(1)
• Pub. Date : 2025
• Page : pp.e41319
• Keyword :